澶嶅埗鎴愬姛
  • 鍥炬鑳屾櫙
  • 绾壊鑳屾櫙

绗旇

  • 2019-11-16
    涓哄ぇ浜哄甫鏉ュ舰璞$殑缇婄敓鑲栨晠浜嬫潵鍘 涓哄瀛愬甫鍘诲揩涔愮殑鐢熻倴鍥剧敾鏁呬簨闃呰
    璋堣皥鎬庢牱瀛﹀ソ鏁板_鑻忔闈-涓鐢熸枃搴
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涓婁紶浜庯細2015-06-17

绮変笣閲忥細25

极速快三彩票平台—官方网址22270.COM瓒佺幇鍦ㄧ殑鍙屾墜杩樿兘鎷ユ姳褰兼锛岃秮鎴戜滑杩樿兘鍛煎惛锛屽幓瑙佷綘鎯宠鐨勪汉锛屽仛浣犳兂鍋氱殑浜嬨

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极速快三彩票平台—官方网址22270.COM鈥擲urvivin鍩哄洜琛ㄨ揪鍙婂叾涓庤儍鐧岄鍚庡叧绯荤殑鍒濇澶存帰璁

涓嬭浇绉垎锛1250

鍐呭鎻愮ず锛 澶嶆棪澶у纭曞+瀛︿綅璁烘枃极速快三彩票平台—官方网址22270.COM鈥擲urvivin鍩哄洜琛ㄨ揪鍙婂叾涓庤儍鐧岄鍚庡叧绯荤殑鍒濇鎺㈣濮撳悕锛氭豹鍏ㄧ孩鐢宠瀛︿綅绾у埆锛氱澹笓涓氾細鍐呯瀛︽寚瀵兼暀甯堬細鐜嬪悏鑰20040414 鑳屾櫙Survi vi n鍩哄洜琛ㄨ揪鍙婂叾涓庤儍鐧岄鍚庡叧绯荤殑鍒濇鎺㈣涓枃鎽樿Survi vi n鍩哄洜涓鸿繎骞存潵鏂板彂鐜扮殑涓涓姉鍑嬩骸鍩哄洜锛岀紪鐮佷竴绉嶆柊鐨勪汉浣撶粏鑳炲噵浜℃姂鍒惰泲鐧( hum ani nhi bi torofapoptosi s锛宧IAP) 锛屾槸鎶戝埗缁嗚優鍑嬩骸鐨勯噸瑕佹垚鍒嗭紝鍏舵姂鍒朵綔鐢ㄨ繙杩滆秴杩嘼cl 涓2锛屾槸杩勪粖涓烘鎵鍙戠幇鐨勬渶灏忕殑hIAP铔嬬櫧锛屽湪姝e父鎴愪汉缁勭粐涓竴鑸笉琛ㄨ揪锛岃屽湪澶у鏁版伓鎬ц偪鐦や腑杩囧害琛ㄨ揪銆傚熀浜庡叾鐙壒鐨勭粨鏋勩佺壒娈婄殑缁勭粐鍒嗗竷鍙婃槑鏄剧殑鎶楀噵浜′綔鐢紝survi vi n鍩哄洜鍦ㄦ伓鎬ц偪鐦ゆ棭鏈...

鏂囨。鏍煎紡锛歅DF| 娴忚娆℃暟锛7| 涓婁紶鏃ユ湡锛2015-06-17 22:32:13| 鏂囨。鏄熺骇锛
澶嶆棪澶у纭曞+瀛︿綅璁烘枃极速快三彩票平台—官方网址22270.COM鈥擲urvivin鍩哄洜琛ㄨ揪鍙婂叾涓庤儍鐧岄鍚庡叧绯荤殑鍒濇鎺㈣濮撳悕锛氭豹鍏ㄧ孩鐢宠瀛︿綅绾у埆锛氱澹笓涓氾細鍐呯瀛︽寚瀵兼暀甯堬細鐜嬪悏鑰20040414 鑳屾櫙Survi vi n鍩哄洜琛ㄨ揪鍙婂叾涓庤儍鐧岄鍚庡叧绯荤殑鍒濇鎺㈣涓枃鎽樿Survi vi n鍩哄洜涓鸿繎骞存潵鏂板彂鐜扮殑涓涓姉鍑嬩骸鍩哄洜锛岀紪鐮佷竴绉嶆柊鐨勪汉浣撶粏鑳炲噵浜℃姂鍒惰泲鐧( hum ani nhi bi torofapoptosi s锛宧IAP) 锛屾槸鎶戝埗缁嗚優鍑嬩骸鐨勯噸瑕佹垚鍒嗭紝鍏舵姂鍒朵綔鐢ㄨ繙杩滆秴杩嘼cl 涓2锛屾槸杩勪粖涓烘鎵鍙戠幇鐨勬渶灏忕殑hIAP铔嬬櫧锛屽湪姝e父鎴愪汉缁勭粐涓竴鑸笉琛ㄨ揪锛岃屽湪澶у鏁版伓鎬ц偪鐦や腑杩囧害琛ㄨ揪銆傚熀浜庡叾鐙壒鐨勭粨鏋勩佺壒娈婄殑缁勭粐鍒嗗竷鍙婃槑鏄剧殑鎶楀噵浜′綔鐢紝survi vi n鍩哄洜鍦ㄦ伓鎬ц偪鐦ゆ棭鏈熻瘖鏂侀鍚庡垽鏂強闈跺悜娌荤枟涓殑搴旂敤浠峰艰屽紩璧峰叧娉ㄣ傜洰鐨勬湰鐮旂┒閲囩敤鍏嶇柅缁勭粐鍖栧娉曟娴嬭儍鐧岀粍缁囦腑survi vi n鍩哄洜琛ㄨ揪鎯呭喌锛岄殢璁胯儍鐧屾牴娌绘湳鍚庢偅鑰呯殑鐢熷瓨鐘跺喌锛屽垎鏋愬叾琛ㄨ揪涓庤儍鐧岀梾鐞嗗垎鍖栫▼搴︺佹蹈娑︽繁搴︺佹穻宸寸粨杞Щ鍙婃湳鍚庝簲骞寸敓瀛樼巼涔嬮棿鐨勫叧绯伙紝鎺㈣survi vi n鍩哄洜琛ㄨ揪涓庨鍚庣殑鍏崇郴锛屼粠鍒嗗瓙姘村钩浼拌鑳冪檶棰勫悗銆极速快三彩票平台—官方网址22270.COM鏂规硶鐥呬緥鏉ユ簮浜1995锛7锛1996锛6涓北鍖婚櫌浣忛櫌鎵嬫湳鐥呬汉锛岄噰鐢ㄥ厤鐤粍缁囧寲瀛nvi si on浜屾娉曟娴嬫墜鏈垏闄ょ殑鑳冪檶鐥呯伓缁勭粐涓璼urvi vi n鍩哄洜琛ㄨ揪鍙婂叾p53鍩哄洜琛ㄨ揪銆傝偪鐦ょ殑鐥呯悊绫诲瀷鏍规嵁H E鏌撹壊缁撴灉锛屽苟缁撳悎涓村簥妫鏌ャ佹墜鏈墍瑙佸拰鐥呯悊妫鏌ョ‘瀹氳偪鐦N M 鍒嗘湡銆傛墍鏈夌梾渚嬮殢璁胯嚦灏5骞存垨鑰呯洿鍒版浜°傛瘮杈冧笉鍚岀梾鐞嗙被鍨嬨佷笉鍚孴N M 鍒嗘湡Survi vi n琛ㄨ揪宸紓鍙婂叾涓巔53鍩哄洜琛ㄨ揪鐨勭浉鍏虫с极速快三彩票平台—官方网址22270.COM鎸塖urvi vi n鍩哄洜鏄惁琛ㄨ揪姣旇緝涓ょ粍鐢熷瓨鏇茬嚎鐨勫樊寮傘傝鏁拌祫鏂欏寘鎷琒urvi vi n琛ㄨ揪閲囩敤鐜囨垨鏋勬垚姣旀弿杩帮紝姣旇緝鑳冪檶涓嶅悓涓村簥鍒嗘湡銆佹蹈娑︽繁搴﹀強鏈夋棤娣嬪反缁撹浆绉荤粍涓璼urvi vi n鍩哄洜琛ㄨ揪鐜囧樊寮傞噰鐢▁ 2妫楠岋紝鐢熷瓨鍒嗘瀽閲囩敤瀵垮懡琛ㄦ硶锛屼袱缁勬瘮杈冮噰鐢↙ogrank妫楠屻 缁撴灉鍏辨湁96渚嬫偅鑰呰繘鍏ョ爺绌跺拰闅忚瑙傚療銆傚叾涓敺鎬59渚嬶紝濂虫37渚嬶紱骞撮緞鏈灏29宀侊紝鏈澶84宀侊紝骞冲潎56宀侊紱鑲跨槫鍙戠敓浜庤儍搴22渚嬨佽儍浣35渚嬨佽儍绐35渚嬶紝绱強2涓垨浠ヤ笂閮ㄤ綅4渚嬶紱鐥呯悊绫诲瀷涓吅鐧78渚嬶紝闈炶吅鐧18渚嬶紝鍖呮嫭绮樻恫鑵虹檶8渚嬶紝鍗版垝缁嗚優鐧4渚嬶紝鏈垎鍖栫棝3渚嬶紝鍙︽湁槌炵檶2渚嬶紝绫荤檶1渚嬶細鐧岀伓灞闄愪簬绮樿啘鍙婄矘鑶滀笅灞13渚嬶紝绱強鑲屽眰18渚嬶紝绱強鍏ㄥ眰63渚嬶紝涓嶈兘鍒ゅ畾娴告鼎娣卞害2渚嬶紱浼存穻宸寸粨杞Щ48渚嬨96渚嬭儍鐧岀粍缁囦腑68渚嬭〃杈緎urvi vi n鍩哄洜浜х墿锛岄槼鎬х巼70锛8锛呫傝儍鐧岀殑涓嶅悓鐥呯悊绫诲瀷Survi vi n鍩哄洜琛ㄨ揪鐩镐技( 鑵虹檶69锛74锛匳S锛庨潪鑵虹檶60锛00锛咃紝p=O 锛369) 锛岃吅鐧屼腑灞闄愪簬绮樿啘鍙婄矘鑶滀笅灞傜粍Survi vi n鍩哄洜琛ㄨ揪浣庝簬鍥烘湁鑲屽眰娴告鼎鍙婃祮鑶滃眰娴告鼎缁(33锛33锛匳S锛81锛25锛& 75锛93锛咃紝p=O 锛020)銆備即鏈夋穻宸寸粨杞Щ鐨勭梾渚嬮槼鎬х巼涓庢棤娣嬪反缁撹浆绉荤殑鐥呬緥鏃犲樊寮( 68锛09锛匳S锛70锛83锛咃紝p=O 锛771)銆傝吅鐧屼腑楂橈綖涓垎鍖栫粍浣庝簬浣庡垎鍖栫粍(62锛50锛匳S锛82锛05锛咃紝p=O 锛053)锛岃孡ogi sti c鍥炲綊鍒嗘瀽琛ㄦ槑survi vi n鍩哄洜琛ㄨ揪浠呬笌鑳冭吅鐧屾蹈娑︽繁搴︽湁鍏( p<O 锛05) 銆係urvi vi n鍩哄洜琛ㄨ揪涓巔53琛ㄨ揪鏃犵浉鍏虫с68渚媠urvi vi n闃虫ц〃杈剧殑鐥呬緥浜斿勾鐢熷瓨鐜囦负42锛93锛咃紝浣庝簬28渚媠urvi vi n闃存х粍浜斿勾鐢熷瓨鐜( 52锛93锛) 锛宲=O 锛419锛涚敓瀛樺垎鏋愭樉绀洪槼鎬х粍涓庨槾鎬х粍鐢熷瓨鏇茬嚎鍦ㄧ梾绋嬫棭鏈熸棤宸紓锛屽悗鏈熸笎鏄惧樊寮傘傜粨璁簊urvi vi n鍩哄洜鍦ㄨ儍鐧屼腑杩囧害琛ㄨ揪锛涘叾琛ㄨ揪涓庤儍鑵虹檶娴告鼎娣卞害鍙婂垎鍖栫▼搴︾浉鍏抽槼鎬х粍涓庨槾鎬х粍鐢熷瓨鏇茬嚎鍦ㄧ梾绋嬫棭鏈熸棤鏄庢樉宸紓锛屽悗鏈熸笎鏄惧樊寮傘极速快三彩票平台—官方网址22270.COM鍏抽敭璇嶏細鑳冪檶survi vi n鍩哄洜棰勫悗鍏嶇柅缁勭粐鍖栧 Survi vi nExpressi oni n G astri c Cancerous Ti ssue and ItsRel ati onshi pw i thPrognosi sAbstractBackgroundSurvi vi n锛宼hesm al l est hum an i nhi bi tor ofapoptosi s protei n(M AP)’ i sone novelm em ber of anti -cancerousgene fam i l y(foundi n1997)锛嶴urvi vi ni s notexpressedi nnorm al adul t ti ssues but i nm al i gnant tum or锛嶴urvi vi n锛寃 hi chi s si m i l ar w i thp53锛宧asi nduced thestudyi nterestsonearl y di agnosi sofm al i gnant tum or, prognosi sj udgem entandtarget therapy锛0bj ecti vesO urstudyi s toexpl oretherel ati onshi pbetw een survi vi nexpressi onandgastri ccancerprognosi s锛嶪m m unohi stochem i ealm ethodw as used to detect theexepressi onofsurvi vi ngenei ngastri ccarci nom a ti ssues resected fromgastri ccarci nom apati entsw i th curati ve tum or resecti on锛嶣ased on these resul ts锛巜 eanal ysedtherel ati onshi pbetw een theexepressi onof survi vi n andpathol ogi cal grade锛宑l i ni cal stageand5涓yearSBIVi val rate锛嶮 ethodsAl lthesam pl esw ere col l ected frompati ents w ho w eredi agnosedasgastri ccancer and underw entsurgeryi nShanghal ZhongShanH ospi talfromJ ul y1995 toIm m une—hi sto-chem i calanal ysi sm ethod“ Envi si on tw osteps” w asusedto detect survi vi n andp53 expressi oni n the resectedgastri ccancerous ti ssue锛嶤l i ni calstageW aS determ i nedby pathol ogi cal di agnosi sand di ni cal status锛嶢l l the cases w erefol l ow edupat l east 5yearsor censoredbydeath锛嶴urvi vi nexpressi ondi fferenti ati onw erecom paredi n di fferentpathol ogi ca[ types锛宒i fferentTN Mstagesand al so theJ une 1996锛巖el ati vi tyw i thp53w as studi ed锛嶴urvi valcurve w ascom paredi n the tw ogroupsw hether survi vi n w asexpressed锛嶳esul tsTotal l y96caSeS(m al e锛歠em al e锛59锛37锛沘ge29—84years ol d锛宮 eanage锛56 yearsol d) w ererecrui ted锛嶵he l ocati on denoted aS锛22 caSes i nfundus锛35 cases i nbody,3 35 cases i n si nus锛4cases w i th m ore than tW Ol ocati ons锛嶱athol ogi cal l y, 78casesw ere adenocarci nom a锛18 w ere non—adenocarci nom a i ncl udi ng 8 cases of m ucusadenocarci nom a锛4cases ofri ngcel l ul ar carci nom a锛3cases of undi fferenti atedcarci nom a锛2 casesof銆乻quam ous carci nom a锛1 case of carci noi dcarci nom a锛13 casesw ere l i m i ted to m ucosa andsubm ucoca锛18 cases l i m i ted to m uscul ari sl ayeL63 casesi nfl i trated to al l thel ayers锛2cases cannot be determ i ned锛48 cases w i ml ym phoi dm etastasi s锛嶵otal survi vi nexpressi onrate W as 70锛8锛卹68锛96)锛嶴i m i l ar expressi onratesexi sted i n di fferent pathol ogi cal types( adenocarci nom a VS锛嶯 on- adenocarci nom a锛69锛74锛匳S锛60锛00锛咃紝p20锛369)锛孲urvi vi n expressi onrate w ashi gheri n the ti ssue w i thdeep l ayersi nfi l trated than thatsuperfi ci al l y i nfi l trated(1i m i ted to m ucosa andsubm ucosa vs锛1i m i ted to m uscul ari sl ayer& i ni l i tratedto al l thel ayers锛33锛33锛匳S锛81锛25锛& 75锛93锛咃紝p=O 锛020銆侊紟Expressi onrate w as nothi gheri n cases w i thl ym phoi dsm etastasi s thannon—l ym phoi ds m etastasi s(68锛09锛匳S锛70锛83锛咃紝p=O 锛771)锛嶴urvi vi nexpressi onw as not rel ated top53 expressi on锛5- yearsurvi valexpressedcases w as 42锛93锛咃紝w hi chw as l ow er than that of 28 cases w i thout survi vi nrate i n 68 survi vi nexpressed(52锛93锛咃紝p=O 锛419)锛嶪nsurvi val curve锛宼hereW as not asi gni fi cant di fferencei n theearl y stage( 2years) but aseperati onafter tw oyearsbetw eensurvi vi n锛巔osi ti vegroupandsurvi vi n· negati ve group锛巓onCl U Sl onSSurvi vi n w ashi ghl y expressedi ngastri ccancer and rel ated w i th Tstageandpathol ogi cal grade锛宐ut not w i th Nstage锛宲athol ogi cal typeandp53 expressi on锛嶪nsurvi val cl l rve锛宼here w as not asi gni fi cant di fference i n theearl y stage(2years)butaseperati on after tw o years betw eensurvi vi n-posi ti ve groupandsurvi vi n—negati vegroup锛嶬eyW ords锛欸 astri c carci nom aSurvi vi nIm m unohi stochem i stryPrognosi s4 寮曡█鑳冪棝鍙戠梾鐜囧強姝讳骸鐜囧湪鎭舵ц偪鐥涗腑鍒嗗眳涓銆佷簩浣嶁嫰锛屼弗閲嶅▉鑳佺潃浜虹被鐨勫仴搴枫備粠澶т綋褰㈡佸強鐥呯悊缁嗚優瀛︽按骞冲彲澶ц嚧鍒ゆ柇棰勫悗鎯呭喌銆傞殢鐫鍒嗗瓙鐢熺墿鎶鏈殑鍙戝睍锛屼汉浠愭鍙戠幇涓棘褰㈡佸姘村钩鏈夋椂涓嶈冻浠ュ噯纭湴棰勬祴棰勫悗锛岃屽垎瀛愭按骞崇殑鐮旂┒鍙笌褰㈡佸鐩镐簰琛ュ厖锛屾彁渚涗竴涓洿涓哄畬鍠勭殑璇勪环浣撶郴12J 锛屼粠鑰屾洿鍔犵簿纭湴鍒ゆ柇棰勫悗锛屼负涓村簥閫夋嫨鍚堢悊鐨勫共棰勬帾鏂芥彁渚涚悊璁轰緷鎹傜洰鍓嶇爺绌惰涓猴紝鑳冪檶鏄鍥犵礌缁煎悎浣滅敤鐨勭粨鏋滐紝鍏跺彂鐥呬笌閬椾紶鍥犵礌銆侀ギ椋熶範鎯佷簹纭濊兒绫诲寲瀛﹁嚧鐧岀墿鎽勫叆鍙婂菇闂ㄨ灪鏃嬫潌鑿( H el i cobacterPyl ori 锛孒 p) 鎰熸煋鏈夊叧锛屽寘鎷绉嶇檶鍩哄洜婵娲诲拰鎶戠檶鍩哄洜鐨勫け娲汇備竴鑸涓猴紝鑳冪檶鐨勫彂鐢熼伒寰“ 鎱㈡ф祬琛ㄦц儍鐐庝竴鎱㈡ц悗缂╂ц儍鐐庝竴鑲犱笂鐨寲鐢熶竴涓嶅吀鍨嬪鐢熶竴鑳冪檶” 杩欎竴杩涘睍妯″紡銆3]锛屽苟涓庡绉嶅熀鍥犵獊鍙樻湁鍏筹細( 1) 绐佸彉鍨媝53鍙奲cl 涓2锛屽垎鍒綔鐢ㄤ簬缁嗚優鍑嬩骸鐨勪笉鍚岀幆鑺傦紝鎶戝埗缁嗚優鍑嬩骸锛屼笌鑲跨槫鐨勫彂鐢熴佸彂灞曞強渚佃鐩稿叧锛( 2) Ras鍩哄洜绐佸彉锛屽彲褰卞搷鍏朵骇鐗㏄21姘磋ВG TP( 涓夌7閰搁笩鑻) 鐨勮兘鍔涳紝鏈缁堝彲瀵艰嚧鐧屽彉锛( 3) ri m 23鍩哄洜锛屾槸涓绉嶆柊鍙戠幇鐨勮璁や负鏄偪鐦よ浆绉绘姂鍒跺熀鍥狅紝鍦ㄦ伓鎬ц偪鐦ょ粍缁囦腑鍑虹幇绛変綅鍩哄唸鐨勭己澶憋紝瀵艰嚧鑲跨槫鐨勮浆绉伙紱( 4) GF( 鐢熼暱鍥犲瓙) 瀹舵棌锛屼績杩涚粏鑳炲鍊笺佸垎瑁傘佸垎鍖栵紝涓庤偪鐦ょ殑鍙戠敓鍙婁镜琚湁鍏炽傝儍鐧屼腑EG F( 琛ㄧ毊鐢熼暱鍥犲瓙) 銆乂EG F( 琛绠″唴鐨敓闀垮洜瀛) 鍙奣G F( 杞寲鐢熼暱鍥犲瓙) 鍧囪繃搴﹁〃杈撅紱( 5) E—cadheri n( 閽欑矘绱) 涓轰竴绉嶇矘闄勫洜瀛愶紝鍏剁粨鏋勫強鍔熻兘鍧囦緷璧栭挋绂诲瓙锛岃兘澶熸姂鍒剁粏鑳炵敓闀匡紝鍏跺湪鑲跨槫涓〃杈句笅璋冧笌鑲跨槫鐨勪镜琚垚姝g浉鍏炽係urvi vi n鍩哄洜涓1997骞存柊鍙戠幇鐨勪竴涓姉鍑嬩骸鍩哄洜锛孉m bosi ni G14銆戦娆℃姤绂忓缓蹇笁鎶宸 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n鍩哄洜涓昏瀛樺湪浜庣粏鑳炴祮涓紝鑰屼笖survi vi n鍩哄洜鐨勪綔鐢ㄤ綅鐐筩apase( 鍗婅儽姘ㄩ吀閰) 涔熷瓨鍦ㄤ簬缁嗚優娴嗭紝鐩墠澶氳涓簊urvi vi n鍩哄洜鍦ㄧ粏鑳炴祮涓彂鎸ユ姂鍒剁粏鑳炲噵I=鐨勪綔鐢ㄣ傝嚦浜庡叾纭垏鐨勪綔鐢ㄦ満鍒跺皻涓嶅崄鍒嗘槑纭傚熀浜巗urvi vi n鍩哄洜鐙壒鐨勭粨鏋勩佺壒娈婄殑缁勭粐鍒嗗竷鍙婃槑鏄剧殑鎶楀噵浜′綔鐢紝鍏跺湪鎭舵ц偪鐦ゆ棭鏈熻瘖鏂侀鍚庡垽鏂強闈跺悜娌荤枟涓殑搴旂敤浠峰艰屽紩璧峰叧娉ㄣ傚浗鍐呭鐮旂┒鍧囨樉绀哄叾涓庢伓鎬ц偪鐦ゅ彂鐢熺浉鍏砙15-17]銆傝嚦浜庢槸鍚︿笌棰勫悗鏈夊叧锛屾姤绂忓缓蹇笁鎶宸 鈥旈椤-涓峗銆18銆20銆戙傚叧浜庡叾鍦ㄨ儍鐧屼腑琛ㄨ揪涓庤儍鐧屾偅鑰呯敓瀛樼姸鍐典箣闂寸殑鍏崇郴锛屽湀鍐呯浉鍏虫姤绂忓缓蹇笁鎶宸 鈥旈椤-涓嶅銆傛湰鐮旂┒閲囩敤鍏嶇柅缁勭粐鍖栧娉曟娴嬭儍鐧岀粍缁囦腑survi vi n鍩哄洜琛ㄨ揪鎯呭喌锛岄殢璁胯儍鐧屾牴娌绘湳鍚庢偅鑰呯殑鐢熷瓨鐘跺喌锛屽垎鏋愬叾琛ㄨ揪涓庤儍鐧岀梾鐞嗗垎鍖栫▼搴︺佹蹈娑︽繁搴︺佹穻宸寸粨杞Щ鍙婃湳鍚庝簲骞寸敓瀛樼巼涔嬮棿鐨勫叧绯伙紝鎺㈣survi vi n鍩哄洜琛ㄨ揪涓庨鍚庣殑鍏崇郴锛屼粠鍒嗗瓙姘村钩浼拌鑳冪檶棰勫悗锛屽悓鏃舵娴嬪拰鍒嗘瀽P53鍩哄洜琛ㄨ揪鍙婂叾涓庝复搴婄梾鐞嗚仈绯伙紝浠庡垎瀛愭按骞充及璁¤儍鐧岄鍚庛6 鏉愭枡鍜屾柟娉曚竴锛庣梾渚嬮夋嫨涓庝复搴婇殢璁跨梾渚嬫潵婧愪簬涓北鍖婚櫌1995锛7锝1996锛6浣忛櫌鎺ュ彈鑳冪檶浜庢湳鎮h呮昏203渚嬶紝鍏ラ夋湰鐮旂┒鏍囧噯锛( 1) 鑳冪檶鏍规不鏈( 濮戞伅鎬у垏闄ゅ強鎺㈠姟鏈櫎澶) 锛( 2) 鏈墠鍧囨湭鎺ュ彈鏀俱佸寲鐤椼傛渶鍚96渚嬫偅鑰呯鍚堝叆閫夋潯浠惰繘鍏ユ湰鐮旂┒锛屼粬浠潎鏈夎缁嗙殑鎵嬫湳璁板綍鍙婄梾鐞嗚祫鏂欙紝鍖呮嫭绫嶈疮銆佸勾榫勩佹у埆銆佽亴涓氥佹墜鏈柟寮忋佹湁鏃犺繙澶勮浆绉汇佺梾鐞嗗垎鍨嬨佺粍缁囧垎鍖栥佹蹈娑︽繁搴﹀強鏈夋棤娣嬪反缁撹浆绉汇俿urvi vi n鍙妏53鍩哄洜琛ㄨ揪閲囩敤鍏嶇柅缁勭粐鍖栧鏂规硶璇勪环锛屾墍鏈夌梾浜哄潎鏈夋湳鍚庣粍缁囩暀瀛樼殑铚″潡锛屾瘡渚嬫偅鑰呴噸鏂拌繛缁垏鐗囧洓寮狅紝涓寮犵敤浜嶩 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鏁d负闃虫у鐓э紝TBS缂撳啿娉唬鏇匡綖鎶楀仛涓鸿功鎬у鐓с傚叚锛庣粨鏋滆瀵熺炕缁囪矮鐗囨煋鑹插悗璧㈠嶉暅F瑙傚療f閲囧鐩叉硶璇荤墖锛岄儙璋堢墖鏃朵笉鐭ョ寤哄揩涓夋妧宸 鈥旈椤-璇ユ偅鑰呯殑涓村簥鎯呭喌) 锛屽彇5涓閲庯紝鎬昏鏁500涓粏鑳烇紝survi vi n鏄剧ず鑲跨槫缁嗚優娴嗙潃鑹叉丁闃虫э紝p53鏄剧ず缁嗚優鏍告贰榛勮嚦妫曢粍涓洪槼鎬х粏鑳烇紝鍏朵腑鐫鑹插己搴︾Н鍒嗙幉j 锛0=鏃犵潃鑹诧紝l =缂ㄧ(娴嗘垨缁嗚函鏍歌鏁欏湪缇炶壊棰楃矑锛2=缁嗚吙娴嗘垨缂ㄨ吙鏍歌灏戦噺缇炶壊榛荤矑锛3涓瑙佸ぇ鎳跨潃鑹查绮掞紱鐫鑹茬粏鑳炴瘮渚嬬Н鍒嗭細0涓鐫鑹叉瘮渚嬪皬浜5锛呫乴 =5-24锛呫2=25锝49锛呫3—50锝74锛呫4=75锛呮畫娴氫笂锛涙爣璁板垎鏁=钃胯壊寮哄害× 铔拌壊璺戦暃锛屾爣璁板垎鏁板皬浜1灞0绾э紝l 锝3灞瀕 绾э紝4锝6灞2绾э紝7锝9灞3绾э紝10锝12灞4绾с傝〃2鍑柅缁勫寲缁撴灉鍒ゅ畾鏍囧噯9 涓冿紟H E鏌撹壊姣忎緥甯歌琛孒 E鏌撹壊澶嶈瘖锛岀敱鐥呯悊绉戣祫娣卞尰涔嶈鐗囪瘉瀹炲垏鐗囦腑鐧岀粏鑳炶兘婊¤冻鍏嶇柅缁勫寲瀹為獙鐨勯渶瑕併傚叓锛庣粺璁″垎鏋愬皢鎵鏈夎祫鏂欏潎杈撳叆璁$畻鏈猴紝搴旂敤spssl 1锛5缁熻杞欢杩涜鏁版嵁澶勭悊銆傝鏁拌祫鏂欏寘鎷琒urvi vi n琛ㄨ揪閲囩敤鐜囨垨鏋勬垚姣旀弿杩帮紝姣旇緝鑳冪檶涓嶅悓涓村簥鍒嗘湡銆佹蹈娑︽繁搴﹀強鏈夋棤娣嬪反缁撹浆绉荤粍涓璼urvi vi n鍩哄洜琛ㄨ揪鐜囧樊寮傞噰鐢▁ 2妫楠岋紝鐢熷瓨鍒嗘瀽閲囩敤瀵垮懡琛ㄦ硶锛屼袱缁勬瘮杈冮噰鐢↙og rank姒嗛獙銆 缁撴灉涓銆傜棸渚嬬殑涓鑸祫鏂96渚嬩腑鐢59渚嬶紝濂37渚嬶細骞撮緞鏈灏29锛1宀侊紝鏈澶83锛5宀侊紝骞冲潎涓綅骞撮緞56锛4宀侊細鍙戠敓浜庨搴曘佷綋銆佺鍒嗗墣涓22銆35銆35{鍔砵 锛屽閮戒綅4閾★紱楠х槜鐮栨伝锛岀矘娑茶吅鐧8渚嬶紝鍗版垝缁嗚優鐧4渚嬶紝槌炵檶2渚嬶紝绫荤檶1渚嬶紝瀹嬪垎鍖栫檶3渚嬶紱浼存穻鏆囩粨杞Щ49閾★紝钀佷綑47鍢茬瓲鍙戠幇鑰卞お娣嬪反缁撴噵鏈剦鐦哥悊鏈虎鐜扮檶缂佹柦锛涚樃鐏跺眬榛讳簬绮樿啘鍙婄矘鑶滀笅灞13渚嬶紝绱強鑲屽眰18渚嬶紝绱強鍏ㄥ眰63渚嬶紝涓嶈兘鍒ゅ畾娴告鼎娣卞害2渚嬶細鐥呯悊鍒嗗寲鎸塀l oom —Ri 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2妫楠屽強Fi sher妫楠屽垎鏋恠urvi vi n鍩哄洜涓庤儍鐧屽彂鐢熼儴浣嶃佺粍缁囧垎鍨嬨佹蹈娑︽繁搴︺佺梾鐞嗗垎鍖栫▼搴﹀強鏈夋棤娣嬪反缁撹浆绉婚棶鐨勫叧绯伙紝鎸塻urvi vi n鍩哄洜琛ㄨ揪鍒嗚〃杈俱佷笉琛ㄨ揪浜岀粍锛屽叾涓笉琛ㄨ揪涓0绾э紝琛ㄨ揪鍖呮嫭1銆2銆3鍙4绾э紱鍙戠敓閮ㄤ綅鍒嗕负鑳冨簳璐查棬銆佽儍浣撱佽儍绐﹀強澶氶儴浣4缁勶紱缁勭粐鍒嗗瀷鍒嗕负鑵虹檶鍙婇潪鑵虹檶缁勶紱鑵虹檶娴告鼎娣卞害鍒嗙矘鑶滃強绮樿啘涓嬪眰銆佽倢灞傚強娴嗚啘灞備笁缁勶紝鍙﹀2渚嬫蹈娑︽繁搴︿笉鑳藉垽瀹氳呭垎鏋愪腑寮冨幓锛涜吅鐧岀梾鐞嗗垎鍖栫▼搴﹀垎涓洪珮锝炰腑鍙婁綆鍒嗗寲浜岀粍锛涙穻宸寸粨杞Щ鍒嗕负鏈夋棤浜岀粍锛涚粨鏋滄樉绀虹矘鑶滃強绮樿啘涓嬪眰娴告鼎缁勯槼鎬х巼鏄庢樉浣庝簬鑲屽眰娴告鼎鍙婃祮鑶滃眰娴告鼎缁( 33锛33锛匳S锛81锛25锛咃紗75锛93锛咃紝p=0锛020) 锛岄珮涓涓垎鍖栫粍鏄庢樉浣庝簬浣庡垎鍖栫粍(62锛50锛匳S锛82锛05锛咃紝p=0锛053)锛岃〃4銆傝〃4survi vi n琛ㄨ揪鐩稿叧鍥犵礌鍒嗘瀽鍙戠敓閮ㄤ綅N =96鑳冨簳璐查棬鑳冧綋鑳冪澶氶儴浣嶇粍缁囧垎鍨婲 =938鑵虹檶闈炶吅鐧16222635496139124672锛72锛0锛735’62锛86锛74锛29锛75锛巓o锛69锛74锛0锛36960锛00锛呰吅鐧屽垎鍖朜 =78楂樸佷腑浣251562锛50锛匫 锛05331782锛05锛呰吅鐧屾蹈娑 =78绮樿啘鍙婄矘鑶滀笅灞傚浐鏈夎倢灞傛祮鑶滃眰3633锛33锛0锛02013381锛25锛401375锛93锛呮穻宸寸粨杞ЩN =96F—__浜岀敓——————鍏癓锛!!’ Fi sher妫楠屾湁331668锛09锛0锛771ZQ锛氬澧82渚嬮碁鐧岋紝1渚嬬被鐧屾湭浜堝垎鏋17 浜旓紟Survi vi n鍩哄洜琛ㄨ揪鐨勫鍐堣雹鍒嗘瀽杩愮敤Logi sti c鍥炲綊瀵箂urvi vi n鍩哄唸琛ㄨ揪涓庤儍鑵虹檶娴告鼎娣卞害銆佺梾鐞嗗垎鍖栫▼搴﹀強鏈夋棤娣嬪反缁撹浆绉荤瓑杩涜澶氬唸绱犲垎鏋愬緱琛5锛屼粠琛ㄤ腑鍙互鐪嬪嚭survi vi n鍩哄洜琛ㄨ揪浠呬笌鑳冭吅鐧屾蹈娑︽繁搴︽湁鍏( p<0锛05) 锛岃屼笌鑵虹檶鐥呯悊鍒嗗寲绋嬪害鍙婃湁鏃犳穻宸寸粨杞Щ鏃犲叧( p>0锛05) 銆傝〃5survi vi n琛ㄨ揪澶氬洜绱犲垎鏋愬洜绱燘S锛嶦锛嶱鍊糘 R娴告鼎娣卞害涓0锛791 10锛3700O 锛0325O 锛4533娣嬪反缁撹浆绉讳竴O 锛72070锛55170锛19140锛4863鍒嗗寲绋嬪害0锛7332O 锛5842O 锛1 8592锛1 657鍏紟鑳冪檶缁勭粐涓璓53鍩哄洜鐨勮〃杈96渚嬩腑39渚嬭p53鍩哄洜琛ㄨ揪锛岄槼鎬х巼涓40锛6锛咃紝闃虫х▼搴0銆1銆2銆3鍙4鍒嗗埆鍗犳绘暟鐨59锛4锛呫14锛6锛呫5锛2锛呫6锛3锛呭強14锛6锛咃紝鍏蜂綋鎯呭喌瑙佽〃6鍙婂浘9銆侾53鍩哄洜琛ㄨ揪闃虫ф煋鑹蹭富瑕佸畾浣嶄簬鑲跨槫缁嗚優鏍镐腑锛屼负娣¢粍鑷虫榛勮壊棰楃矑锛岃( 鍥10锝11銆併 閬! 鑺峰缇归瓐鏌ユ槦钑夌敓鑹樺鍨勯」鐩▼搴︾梾渚嬫暟鎺忔垚姣旇簮9P53钘诲洜鍦ㄨ噣鐧岀紑缁囦腑闃抽珛琚揪鍒嗗竷19 璞10P53闃虫( 鑵虹檶锛岀粏鑳炴牳绯熻壊锛400X)鍥11P53闃虫( 浣庡垎浠栬吅鐧岋紝缁嗚優姊潃鑹诧紝400X) 涓冿紟榛3鍩哄洜琛ㄨ揪鐨勮鍏冲洜绱犲垎鏋愬簲鐢≒earsonx2妫楠屽強Fi sher妫楠屽垎鏋怭53鍩哄洜琛ㄩ镐笌鑳冪樃鍙戠敓閮ㄤ綅銆佺粍缁囧垎缇广佹蹈婵戞繁搴︺佺椏鐞嗗垎绾▼鍙戝強楦棤娓埁缂濊浆绉婚槙澶斿叧绯伙紝缇53鍩哄洜琛ㄨ揪鍒嗚〃杈俱佷笉琛ㄨ揪浜岀粍锛屽叾涓笉琚揪涓0绾э紝琛ㄨ揪鍖呮嫭1銆2銆3鍙4绾э紱鍙戠敓閮ㄤ綅鍔充负闇勮耽榧楅棬銆佽法娑斻佽ぅ绐﹀強澶氶儴浣4缁勶紱缂缂撳垎閸垎蹇呰鲍鐧屽強鑿茶吅缁辩及锛涜鲍鐧屾蹈娑︽繁搴﹀垎绮樿啘鍙婄矘鑶滀笅灞傘佹箹鏈夎倢灞傚強娴嗚啘灞備笁缁勶紝鍙﹀2渚嬫蹈娑︽繁搴︿笉鑳藉垽瀹氳呭垎鏋愪腑寮冨幓{鑵虹檶绮剧悊鍒嗗寲绋嬪害鍒嗕负楂橈綖涓強浣庡垎鍖栦簩缁勶紱娣嬪反缁撹浆绉诲垎涓烘湁绗间簩缁勶紱浠庤〃涓彲浠ラ簱鍑篜53鍩哄洜鎲嬭〃杈句笌娴告汉娣卞害銆佺樃鐞嗗垎鍖栫▼搴﹀強鏈夌娓反缂╄浆绉婚棿鏃犳槑椹荤浉鍏虫( p銆0锛05锛屽瘽7) 銆傝〃7p53琛ㄨ揪瑜跺叧鍥犵礌鍒嗘瀽鍙戝斗閮ㄤ綅N =96鑳冨簳璐查棬楂綋鑳冨癁澶氶兘绡穕 Ol 閲16鍦112519347锛62锛0锛236+30銆56锛45锛71锛25銆∞锛呯粍缁囧垎鍨婲 =938鑹ㄧ檶闈炶吅鐧33姣54l 銆98锛呫0锛53151033锛33锛呰鲍鐥垮姫寰種 =78鍟嗐佷腑浣1525∞37锛50锛0锛3781847锛37锛呰吅鐦告蹈娑 =78宓囩皣鍙婅矘涓嬮鍥烘湁鑲屽眰娴嗚啘灞3537锛50锛0锛39951033锛33锛233241锛07锛呮穻宸寸粨杞ЩN =96-—————钄撲箼———涓涓锛庡⒓锛庝竴82閾¢碁鐧岋紝1闀勭被鐦瘨浜堝垎鎺囨湁193039锛58锛0锛769鍨笣锛氫富澧ㄥ銆侳i sher妫楠 鍏傞湁鐧岀紑缁囦腑survi vi n涓嶱53鍩哄洜琚揪鐨勭浉鍏崇儹鎴戜滑瀵箂urvi vi n鍩哄洜琛ㄨ揪涓巔53鍩哄洜琛ㄨ揪鐨勭浉鍏虫ц繘琛屼簡璇勪环锛屾柟娉曚负璁$畻鑴氬煎潻娆哄垝鍦充负鑴” 鎺g剦绔芥慨涓窞榛艰幊鏍兼潈鐔忥紝Poi j 涓虹i j 鏍煎疄闄呬竴鑷存瘮锛孭ei j 涓虹i j 鏍兼湡鏈涗竴鑷存瘮銆傝垎浣撴儏鍐佃琛8锛孠appa鍊间负0锛067( Kappa<0锛2鎻愮ず浜岃呬竴鑷存т笉鍟) 锛岃〃鏄庝簩鑰呬箣闂存棤鏄庢樉绯叧鎬с傝〃8survi vi n鍩哄尰琛ㄨ揪涓巔53鐩稿叧鎬т節銆傞鍚庤瘎浠锋墍鏈夌樃渚嬪潎璋惰鏈浜斿勾闅忚(鍖呮嫭鏈娌荤枟鎯呭喌)锛屽叾涓92渚嬮殢璁垮畬鏁(51鍊掓湳鍘5骞村唴姝讳簣闇夌檶锛4l 鍊掓椿鐎5骞) 锛4閾″け娓( 鍖呮嵁1閾″瘨铻1锛5骞存鎵嬪績鑴忕梾) 锛屽け璁跨巼3锛9锛咃紝锛30渚嬫湳鍚庢帴鍙楀寲鐤( 5涓FU +M M C锛宭 鐮佺枟绋嬩笉绛) 锛屾湭鍖栫枟12鐖紝6l 閾℃不鐤椾笉璇︺傞噰鐢↘apl an-M ei er缁樺埗鐢熷瓨鐬岀嚎锛屽浘12涓烘讳綋鐢熷瓨鏇茬嚎锛屽浘13涓烘寜survi vi n鍩洪閫跺惁琛ㄨ揪鍒嗙紑鐢熷瓨瑜ョ嚎銆傛讳綋浜斿勾鐢熷瓨鐜囦负48锛1锛咃紝survi vi n闃虫х粍鍙婇槾鎬х粍浜斿勾鐢熷瓨鐜囧垎鍒负42銆93锛2涔52銆93锛咃紝Log Rank鎹¢獙=0锛4186锛涗粠缈13闇逛互鐪嬪嚭survi vi n鍩哄洜琛ㄨ揪鍙婁笉琛ㄨ揪浜岀粍瀚╁瓨鏇茬嚎闂村湪鐦哥▼鏃╂湡鏃犲樊寮傦紝闅忚2骞村悗浜岀収绾垮憟鍒嗚瓒嬪娍銆 鍦12鎱т綋鐢熷瓨铇х紦鍦13榛漟vi vi n鍩哄洜楝绘х紦( 绾㈡) 銆佹埉寰缁( 缁胯壊) 鐢熷瓨璧紳 璁ㄨ杩戝勾鏉ワ紝鍏充簬survi vi n鍩哄洜涓庢伓鎬ц偪鐦ゅ彂鐢熴侀鍚庡強鍩哄洜姘村钩鐨勬不鐤椾箣闂村叧绯荤殑鐮旂┒杩呴熸垚涓哄浗鍐呭鍏虫敞鐑偣鍙2” 銆傞壌浜庡叾鍒簬鍏跺畠hl AP鐨勫垎甯冩ā寮忥細骞挎硾瀛樺湪浜庡悇绉嶆伓鎬ц偪鐦ょ粏鑳炰腑锛岃屽湪姝e父鎴愪汉缁勭粐涓笉琛ㄨ揪锛岀爺绌惰呮璇曞浘闃愭槑鍏跺湪鎭舵ц偪鐦ょ殑鏃╂湡璇婃柇銆侀鍚庣殑棰勬祴鍙婂熀鍑兼按骞崇殑娌荤枟涓婄殑瀹炵敤浠峰笺傚湪浜哄拰榧犵殑鑳氳儙鍙, qffJ L鏈燂紝survi vi n鍩哄洜琛ㄨ揪涓板瘜12銆戯紝鑰屽湪IF甯告垚浜虹粍缁囦腑鍑犱箮鏈娴嬪埌survi vi n鍩哄唸鐨勮〃杈綶 231锛屻傚嚑涔庢墍鏈夌殑鎭舵ц偪鐦ゅ潎杩囧害琛ㄨ揪survi vi n m RN A鍙婅泲鐧借川銆26≈71锛宻urvi vi n鍩哄洜鍦ㄨ偪鐦ょ粍缁囦腑閲嶆柊婵娲荤殑鏈虹悊鐩墠灏氫笉鏄庣‘銆傚繁鏈変笉灏戠爺绌舵姤绂忓缓蹇笁鎶宸 鈥旈椤-survi vi n鍩哄洜鍦ㄨ儍鐧屼腑鐨勮〃杈撅細Lu[ 2] 鍦174渚嬭鑳冪檶鏍规不鏈偅鑰呮湳鍚庤儍鐧岀粍缁囦腑鍙戠幇survi vi n鍩哄洜琛ㄨ揪鐜囦负34锛5锛( 60锛174) 锛岃屽湪鐧屾梺姝e父缁勭粐涓棤琛ㄨ揪銆侰hi ouDo] 鍙戠幇浜哄強灏忛紶姝e父鑳冪矘鑶滀笂鐨粏鑳炶〃杈緎urvi vi n m RN A鍙婅泲鐧斤紝鍏朵腑涓荤粏鑳炪佸缁嗚優鐨勮優鏍稿強鑳炴祮鍧囪琛ㄨ揪锛岃屼笖鑳冪矘鑶滀笂鐨瘮鑳冨井铏圭—鍗滅毊琛ㄨ揪鐜囨洿楂樸俌u銆愬垪鍙戠幇鑳冪檶缁勭粐涓璼urvi vi n m RN A闃虫х巼涓68锛咃紝铔嬬櫧琛ㄨ揪鐜囦负62锛5锛咃紱姝e父鑳冪矘鑶滅粍缁噈 RN A鍙婄浉鍏宠泲鐧藉潎鏃犺〃杈撅紝鑰岀檶鏃佹甯哥粍缁噑urvi vi l 锛弇 RN A闃虫х巼涓22锛咃紝survi vi n铔嬬櫧琛ㄨ揪鐜囦负12锛5锛咃紱杩涗竴姝ョ爺绌跺彂鐜拌儍鐧屾偅鑰呯洿绯讳翰灞炴甯歌儍缁勭粐涓璼urvi vi n m RN A鍙婅泲鐧借〃杈剧巼鍒嗗埆涓轰负27锛呭拰20锛呫俌u浠庝笂杩版暟鍊煎垎鏋愯涓簊urvi vi n m RN A琛ㄨ揪鐜囬珮浜庤泲鐧斤紝浣嗕簩鑰呬箣闂瓨鍦ㄨ繛缁浉鍏虫с俌ang XYIl ” 鍒╃敤RT—PCR瀵硅儍鐧岀粍缁囧強姝e父鑳冪矘鑶滅粍缁囪繘琛宻urvi vi n鍩哄洜搴忓垪鍒嗘瀽鍙戠幇锛岃儍鐧岀粍缁囬珮琛ㄨ揪survi vi n涓4A锛屽叾纭峰熀搴忓垪涓巗urvj vi n瀹屽叏鐩稿悓锛涜屾甯歌儍绮樿啘缁勭粐楂樿〃杈緎urvi vi n涓1B锛屽叾搴忓垪涓彧鏈2涓鏄惧瓙锛岀3涓鏄惧瓙缂哄け銆傛湰瀹為獙涓儍鐧屾梺姝e父缁勭粐涓棤survi vi n鍩哄洜琛ㄨ揪鏀寔杩欎竴瑙傜偣锛屽嵆鐧屾梺姝e父缁勭粐涓璼urvi vi n鍩哄洜鐨勯珮琛ㄨ揪涓鸿鍩哄洜鐨勫彉寮備綋銆俽am m l 28] 鐮旂┒鍙戠幇鍦ㄦ甯镐汉鑳冪矘鑶滅粍缁囦腑survi vi n m RN A寮辫〃杈撅紝铔嬬櫧璐ㄦ按骞虫棤琛ㄨ揪锛涜儍鐧岀粍缁囧強鐧屾梺姝e父缁勭粐楂樿〃杈緎urvi vi n m RN A鍙婅泲鐧斤紝浣嗚儍鐧岀粍缁囩殑琛ㄨ揪姣旂檶鏃佺粍缁囨洿寮虹儓锛氳孋ap锛宒se涓3鐨勮〃杈炬儏鍐典笌survi vi n姝eソ鐩稿弽锛岃鏄巗urvi vi n浣滅敤涓庡噵浜¢斿緞鐨勪笅娓革紝鎶戝埗Capase涓3鐨勬椿鎬с傚椤逛綋澶栫爺绌12” 0J 鏄剧ず鑳冩硨绱犻氳繃涓婅皟鎶楀噵浜″熀鍥爏urvi vi n锛宐cl 涓2鐨勮〃杈捐屾姂鍒剁粏鑳炲噵浜★紝涓庤儍鐧岀殑鍙戠敓鏈夊叧銆傛湰瀹為獙涓儍鐧岀粍缁噑urvi vi n鍩哄洜闃虫ц〃杈剧巼涓70锛8锛咃紝琛ㄨ揪鐜囦笌鍥藉鐮旂┒鐩镐技锛氱檶鏃併嶧甯哥粍缁囨湭琛ㄨ揪璇ュ熀鍥狅紝鑰屼笂杩板浗澶栭儴鍒嗙爺绌禰1037I鍙戠幇鐧屾梺缁勭粐鐢氳嚦 姝e父鑳冪矘鑶滃急琛ㄨ揪survi vi n鍩哄嚩锛屽彲鑳芥湁浠N 绱狅細( 1) SU l l vi vi nm RN A琛ㄨ揪鐜囬珮浜巗urvi vi l l 铔嬬櫧锛( 2) survivi n鍙樺紓浣撶殑浣滅敤鈰' ” 1銆俿tl rv] vi n鍩哄洜鐨勮〃杈句笌鑲跨槫棰勫悗uJ 鑳藉瓨鍦ㄤ竴瀹氬叧绯伙細Sere] a1321鍒嗘瀽144渚嬬粨鑲犵檶鎮h呭彂鐜皊urvi vi n闃虫ф偅鑰呮湳鍚庤骞寸敓瀛樼巼涓53锛咃紝鏄捐憲浣庝簬survi vi n闃存ф偅鑰呯殑77锛7锛咃紝鏁呰涓簊urvi vi n涓洪鍚庝笉鑹殑鐙珛鍗遍櫓鍥犵礌銆俬di da[ 191鍦222渚婤缁嗚優鍨嬫穻宸寸槫涓彂鐜皊urvi vi n闃虫х粍涓庨槾鎬х粍浜斿勾鐢熷瓨鐜囨湁鏄捐憲宸紓( 闃虫х粍VS锛庨槾鎬х粍锛40锛匳S锛54锛咃紝p-0锛02) 銆侽 kada[鍨僱 鍙戠幇鑳冪檶缁勭粐涓粏鑳炴牳鍙婄粏鑳炴祮鍧囪繃搴﹁〃杈緎urvi vi n鍩哄洜锛屽叾涓粏鑳炴牳涓〃杈剧巼涓82锛咃紝缁忓鍙橀噺鍒嗘瀽纭畾涓轰竴鐙珛棰勫悗鍥犲瓙锛屼笌棰勫悗鍛堟鐩稿叧锛屽叾琛ㄨ揪鐜囬殢骞撮緞澧為暱鍙婅绠¤浆绉昏孎闄嶏紝涓洪鍚庤壇濂芥爣蹇楋紱Kap] an M ei er鐢熷瓨鏇茬嚎涔熸湁宸紓锛涚粏鑳炴祮涓〃杈剧巼涓89锛咃紝涓庨鍚庢棤鍏炽傛湰鏂囧彂鐜皊urvi vi n鍩哄洜琛ㄨ揪鍧囧畾浣嶄簬缁嗚優娴嗭紝鑰岀粏鑳炴牳涓棤琛ㄨ揪锛岃冭檻鐧界浜轰笌榛勭浜轰箣闂寸殑鍩哄嚩琛ㄨ揪宸紓鍙兘銆侹ri eg[151鍙戠幇鑳冪檶缁勭粐涓繃搴﹁〃杈緎urvi vi n鍩哄洜鍙婂叾浜岀鍙樺紓浣擄細鈻 EX3鍙--2B锛屼絾浠urvi vi n涓轰富锛屾瘮杈冧笉鍚屼复搴婂垎鏈熻〃杈剧巼鍙戠幇survi vi n鍙娾柍 EX3琛ㄨ揪鐜囦笉闅忎复搴婂垎鏈熸敼鍙橈紝鍙湁锝2B琛ㄨ揪鐜囬殢鍒嗘湡杩涘睍鑰屼笅闄嶏紝鏁匥ri eg璁や负涓2B涓簊urvi vi n鎷姉鍥犲瓙锛屽彲鎶戝埗鍏舵姉鍑嬩骸鑳藉姏銆侺u銆2銆戣涓簊urvi vi n鍩哄洜琛ㄨ揪涓庤儍鐧孨 鍒嗘湡鍛堣礋鐩稿叧锛岃屼笌鐥呯悊鍒嗗寲鍙奣鍒嗘湡鍧囨棤鍏筹紱Yu鍙戠幇survi vi n鍩哄洜鐨勮〃杈句笌鑳冪檶鐥呯悊鍒嗗寲鍙婃穻宸寸粨杞Щ鍧囨棤鍏筹紱鍥藉唴閮ㄥ垎鐮旂┒銆恖 鍙彂鐜皊urvi vi n鍩哄洜琛ㄨ揪涓庤偪鐦ゅ垎鍖栫▼搴﹀強TN M 鍒嗘湡鏈夊叧锛屾晠璁や负survi vi n鍩哄洜琛ㄨ揪涓庤偪鐦ら鍚庢湁鍏筹紝闃虫ц〃杈炬彁绀洪鍚庝笉鑹紱浣嗗浗鍐呭缁濆ぇ澶氭暟鐮旂┒鍧囨湭杩涜鐢熷瓨鍒嗘瀽锛岃屼粎渚濇嵁缁勭粐鍒嗗寲绋嬪害鍙婁复搴婂垎鏈熺瓑鎸囨爣闂存帴鎺ㄦ祴棰勫悗銆傛湰鏂囪缁嗚皟闃呮瘡渚嬬爺绌跺璞$殑鎵嬫湳璁板綍鍙婄梾鐞嗘。妗堬紝鍖呮嫭鑲跨槫鍙戠敓閮ㄤ綅銆佹蹈娑︽繁搴︺佺梾鐞嗙粍缁囧垎鍨嬨佸垎鍖栫▼搴︺佹湁鏃犳穻宸寸粨杞Щ銆俋 2妫楠屾樉绀簊urvi vi n鍩哄洜琛ㄨ揪涓庤儍鑵虹檶鍒嗗寲绋嬪害銆佹蹈娑︽繁搴︾浉鍏筹紝绮樿啘鍙婄矘鑶滀笅灞傛蹈娑︾粍闃虫х巼鏄庢樉浣庝簬鍥烘湁鑲屽眰娴告鼎鍙婃祮鑶滃眰娴告鼎缁( 33锛33锛匳S 81锛25锛& 75锛93锛咃紝p<0锛05) 锛岄珮锝炰腑鍒嗗寲缁勬槑鏄句綆浜庝綆鍒嗗寲缁( 62锛50锛匳S 82锛05锛咃紝p-0锛053) 锛氫絾Logi Sti c鍥炲綊瀵箂urvi vi n鍩哄洜琛ㄨ揪涓庝笂杩板洜绱犺繘琛屽鍥犵礌鍒嗘瀽鍙戠幇璇ュ熀鍥犺〃杈句粎涓庤儍鐧屾蹈娓婃繁搴︽湁鍏( p<0锛05) 锛岃屼笌鐥呯悊鍒嗗寲绋嬪害鍙婃湁鏃犳穻宸寸粨杞Щ鏃犲叧( p>O 锛05) 銆傛湰鏂囩爺绌跺璞″潎杩涜鏈悗浜斿勾浠ヤ笂闅忚锛岀敓瀛樺垎鏋愰噰鐢↘apl an—M ei er鏇茬嚎锛岃鏂规硶缁熻绱鐢熷瓨鐜囷紝瀵逛簩缁勭敓瀛樼巼杩涜杩炵画鎬ф瘮杈冿紝鑰岄潪鏌愪竴鐐圭殑宸紓锛屾晠鎵寰楀嚭鐨勭粨鏋滄洿鍏峰彲闈犳с傜粨鏋68渚媠urvi vi n闃虫ц〃杈剧殑鐥呬緥浜斿勾鐢熷瓨鐜 涓42锛93锛咃紝浣庝簬28渚媠urvi vi n闃存х粍浜斿勾鐢熷瓨鐜( 52锛93锛) 锛屼絾缁熻瀛︽棤宸紓(鍙>O 锛05)锛岀敓瀛樻洸绾挎瘮杈冩樉绀轰袱缁勫湪闅忚鏃╂湡宸紓鏃犳樉钁楁э紝浣嗗湪澶х害鏈悗骞翠簩鑰呮樉绀哄嚭宸紓锛岃繖鍙兘涓庤繘鍏ユ湰鐮旂┒鐨勬牱鏈噺鍋忓皬鏈夊叧锛屾湁蹇呰鎵╁ぇ鏍锋湰閲忥紝浠庤岃繘涓姝ヨ瀵熶簩缁勭敓瀛樼巼涔嬮棿鐨勫樊寮傘係urvi vi n鍩哄洜涓巔53鏄惁瀛樺湪鐩稿叧鎬т篃宸叉湁鎺㈣銆俻53鍩哄洜浣嶄簬17p13锛1锛岀紪鐮佸垎瀛愰噺53000鏍哥7铔嬬櫧锛屼负涓杞綍鍥犲瓙锛屼綔涓篏l 鏈烡N A鎹熶激“ 妫鏌ョ偣” 锛屽綋DN A鍙楁崯锛宲53琛ㄨ揪澧炲姞锛屽鍒跺仠姝紝DN A淇锛屽淇澶辫触锛屽垯瀵艰嚧鍑嬩骸銆331銆侾53鍙洜鐥呮瘨鐧屽熀鍥犵粨鍚堛佺偣绐佸彉銆佺己澶便侀噸鎺掑け娲昏屽け鍘绘姂鐧屼綔鐢紝淇冭繘鎭跺彉銆傜獊鍙樺瀷p53鍦ㄥぇ澶氭暟鑲跨槫涓〃杈句笂璋冿紝鑰屽湪姝e父缁勭粐涓笉琛ㄨ揪锛岀洰鍓嶅璁や负p53鍩哄洜涓庢伓鎬ц偪鐦ら鍚庢棤鍏筹紝鏈枃涔熸湁绫讳技鍙戠幇銆侹ora[8銆戝湪74渚嬩钩鑵虹檶缁勪腑鍙戠幇survi vi n鍩哄洜琛ㄨ揪涓巔53鏈夊叧锛屼絾Tanaka[7]鍦ㄤ钩鑵虹檶缁勪腑锛宻urvi vi n涓巔53琛ㄨ揪鏃犲叧銆傛湰鏂囦腑鍙戠幇survi vi n鍩哄洜鍦ㄨ儍鐧岀粍缁囦腑鐨勮〃杈句笌p53鍩哄洜鏃犳槑鏄剧浉鍏虫с俿urvi vi n鍩哄洜鍜宲53鍩哄洜鍒嗗埆浣滅敤涓庣粏鑳炲噵浜$殑涓嶅悓鐜妭04閾樸1锛庣獊鍙樺瀷p53鍩哄洜浣滅敤涓庣粏鑳炲噵浜¢斿緞鐨勪笂娓革紝鎶戝埗Capase-9鐨勬椿鎬э紱survi vi n浣滅敤涓庡噵浜¢斿緞鐨勪笅娓革紝鎶戝埗Capase涓3鐨勬椿鎬с傛湰鏂囩粨鏋滄彁绀簊urvi vi n鍩哄洜琛ㄨ揪涓巔53鍩哄洜鏃犳槑鏄剧浉鍏虫э紝涔熻〃鏄庝簩鑰呬綔鐢ㄩ斿緞鐨勫樊寮傘傛湰鏂囧彂鐜板湪鑳冪檶涓璼urvi vi n鍩哄洜杩囧害琛ㄨ揪锛屾彁绀哄湪鑲跨槫璧峰闃舵survi vi n鍩哄洜琚縺娲伙紝鍏朵笌p53鍩哄洜琛ㄨ揪鏃犳槑鏄剧浉鍏虫у垯琛ㄦ槑瀹冧滑鍒嗗埆鎶戝埗缁嗚優鍑嬩骸鐨勪笉鍚岀幆鑺傘係urvi vi n鍩哄洜鏄惁涓庤偪鐦ら鍚庢湁鍏筹紝鍥藉唴澶栬鐐逛笉涓锛屾湰鏂囦腑鍙戠幇鍏朵笌鑳冪檶娴告鼎娣卞害瀵嗗垏鐩稿叧锛屼笌鑳冪檶鍒嗗寲绋嬪害鏈変竴瀹氱浉鍏虫э紝浣嗕笌鏈夋棤娣嬪反缁撹浆绉绘棤鏄庢樉鐩稿叧鎬э紝闃虫х粍涓庨槾鎬х粍鐢熷瓨鏇茬嚎鍦ㄩ殢璁垮悗鏈熸笎鏄惧樊寮傘傝嚦浜庡浗澶栦釜妗堟姤绂忓缓蹇笁鎶宸 鈥旈椤-涓彂鐜扮粏鑳炴牳涓〃杈緎urvi vi n鍩哄洜锛屽彲鑳戒负survi vi n鍩哄洜鍙樺紓浣擄紝鏈枃鏃犵浉鍏冲彂鐜帮紝鍊煎緱杩涗竴姝ユ帰璁ㄣ傜敱浜庤繘鍏ユ湰鐮旂┒鐨勬牱鏈噺鍋忓皬锛屽疄楠屾爣鏈潵婧愪簬澶т綋铚″潡锛屼笉鑳借PCR妫娴媘 RN A锛屽姞涔嬫椂闂存湁闄愶紝閮ㄥ垎鏍锋湰鏈埌杈剧爺绌剁粓鐐癸紝鏁呮墍寰楀嚭鐨勭粨璁哄彲鑳藉瓨鍦ㄤ竴瀹氱▼搴︾殑鍋忔併傚鑳藉湪浠婂悗鐨勭爺绌朵腑鍔犲ぇ鏍锋湰閲忥紝寤堕暱闅忚鏃堕棿鐩磋嚦缁堢偣锛屽啀鍔犳祴鏂伴矞鏍囨湰涓璼urvi vi n m RN A锛屽垯鍏剁粨璁烘洿鍏峰彲淇″害銆傚叆閫夋湰鐮旂┒鐨勬牱鏈潎涓烘牴娌绘湳鎮h咃紝鍙繚璇佽緝鐩镐技鐨勮捣鐐瑰拰杈冨厖瑁曠殑闅忚鏃堕棿锛屼絾涓嶅彲閬垮厤閫犳垚閫夋嫨鎬у亸鍊氾紝鎷熻繘涓姝ユ帰璁╯urvi vi n鍩哄洜鍦ㄦ湭鎺ュ彈鏍规不鏈呬腑鐨勮〃杈俱 鏈爺绌舵彁绀虹粨璁1锛巗urvi vi n鍩哄洜鍦ㄨ儍鐧岀粍缁囦腑楂樿〃杈撅紝鑰岀檶鏃佹甯哥粍缁囦笉琛ㄨ揪锛氱檶缁勭粐涓〃杈惧畾浣嶄簬缁嗚優娴嗭紝銆庨潰缁嗚優鏍告湭瑙佽〃杈俱2锛巗urvi vi n鍩哄洜鍦ㄨ儍鐧屼腑鐨勮〃杈句笌鑵虹檶娴告鼎娣卞害瀵嗗垏鐩稿叧锛屼笌鐥呯悊鍒嗙骇鏈変竴瀹氱浉鍏虫э紝鑰屼笌缁勭粐鍒嗗瀷鍙婃湁鏃犳穻宸寸粨杞Щ鍧囨棤鏄庢樉鐩稿叧鎬с3锛巗urvi vi n鍩哄洜鍦ㄨ儍鐧屼腑鐨勮〃杈句笌p53鍩哄洜鏃犲叧銆4· survi vi n鍩哄洜鍦ㄨ儍鐧屼腑鐨勮〃杈句笌鑳冪檶棰勫悗鍦ㄧ梾绋嬫棭鏈熷樊寮備笉鏄庢樉锛岃屽悗鏈熸笎鏄惧樊寮傘 鍙傝冩枃鐚1锛庤惂鏍戜笢涓荤紪銆傛睙缁嶅熀鑳冭偁鐥呭銆備笂娴凤細涓婃捣绉戞妧鍑虹増绀撅紝20012锛嶭uCD锛孉l ti eri D C锛孴ani gaw a N 锛宔t a1锛嶦xepressi onof a novelanti -apoptosi s geneapoptosi s andp53 accum ul ati on i nsurvi vi n correl ated銆佲叄i th tum orcel lgastri ccarci nom a锛嶤ancerResearch锛1998锛58(9)锛1808—18123锛岀帇姊咃紝鐜嬪啺锛岀帇鏅撲附绛夈傚噵浜$浉鍏冲熀鍥爏urvi vi n鍦ㄥ瓙瀹鐧岀粍缁囦腑鐨勮〃杈惧強鍏朵笌bel 涓2锛宲53鍩哄洜琛ㄨ揪鐩稿叧鎬х殑鍒濇鐮旂┒銆備腑鍗庡浜х鏉傚織锛2001锛36(9)锛546—5484锛嶢m brosi ni G锛孉di da C锛孉l ti eriD C锛宔t a1锛嶢 novel anti —apoptosi s gene锛宻urvi vi n锛宔xpressedi n cancer andl ym phorna锛嶯 ature M edi ci ne锛1997锛3(8)锛917—9215锛嶢di daC锛孋rotty PL锛孧 cGrafl a J 锛宔ta1锛嶥 evel opm ental l y regul atedexpressi onof thecancer anti -apoptosi s gene survi vi n i n hum an and m ouse di fferenti ati on锛嶢m eri can J ournal ofPathol ogy锛1998锛152(1)锛43—496锛嶴atohK, Kaneko K锛孒 i rota M 锛宔t a1锛嶦xepressi onof survi vi 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s锛嶤ancerLetters锛2001锛163(1)锛109—11612锛嶬ri eg A锛孧 ahotka C锛孠ri eg T锛宔t a1锛嶦xpressi onof di fferent survi vi n vari ants i ngastri ccarci nom as锛歠i rst cl ues to a rol e of锛2B i n tum orprogressi on锛嶣ri ti sh J ournalofCancer, 2002锛8閰5)锛734—74313锛庨棲姝岋紝榛勭埍榫欙紝鍞愰湏绛夈傜煭鍙戝す鐘禦N A鎶戝埗survi vi n鍩哄洜鍦ㄨ倽鐧岀粏鑳炰腑鐨勮〃杈俱備腑鍗庤倽鑴忕梾鏉傚織锛2003锛11f12)锛712—715 14锛嶩 i roshi Kaw asaki 锛孧 asao Toyoda锛孒 i sashi Shi nohara锛宔t a1锛嶦xepressi onofsurvi vi ncorrel atesw i 灞盿poptosi s锛巔rol i ferati on锛巃nd angi ogenesi s duri nghum an col orectal锛嶢m eri can CancerSoci ety, 2001锛46(5)锛2026—2032tum ori genesi s1 5锛嶢l tura RA锛01shefskipaedi atri c ependym om asand choroi dpl exusturnouts correl ates w i thm orphol ogi ctum ourgrade锛嶣ri ti shJ ournal ofCancer锛2003锛89(9)锛1743—174916锛嶥i m i tri os A锛嶴koufi as锛孋ri sti ana M ol l i nari 锛孎rancoi se B锛宔t a1锛嶩um an survi vi n i s apassengerprotei n锛嶵heJournal ofCel l Bi ol ogy, 2000锛涳細1575-158l17锛庢潹娆h壋锛岀帇瀛熻枃锛-FN U鐭崇瓑銆備汉绫籹urvi vi n鍩哄洜鐨勫厠闅嗗強鍦ㄨ儍绮樿啘缁勭粐涓〃杈綬S锛孞 i ang Y锛宔ta1锛嶯 ucl ear 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unochem i cal l ydi sti nct subcel l ul apool sand i s i nvol l ed i nspi ndl e m ocrotubul efuncti on锛嶫 ournal ofCel lSci ence锛2002锛115锛575锛58525锛嶻oshi noriK锛孎m i ” Iki Y锛孲em i H 锛宔t a1锛嶦xpressi onof Survi vi n i nAstrocyti c Tum ors锛嶤ancer, 2003锛97锛1077—108326锛嶴ej eong S锛孊yungJ 锛嶢nAnti —apoptoti cProtei n H um anSurvi vi n Is aD i rect Inhi bi torofCaspase涓3 and-7锛嶣i ochem i story, 2001锛40锛1117-112327锛嶦dw ardM 锛孲aski a P锛孞 an C锛宔t a1锛嶵hreedi fferenti al l y expressed survi vi n cD N Avari ants encodeprotei ns w i th di sti nctanti —apototi cfuncti ons锛嶣l ood锛2000锛95锛143—145锛 28锛嶵am ml 锛學 ang Y锛孲ausvi l l e E锛宔t a1锛嶪AP—fam i l y protei nsurvi vi n i nhi bi tscaspaseacti vi tyandapoptosi si nducedby Fas锛孊ax锛孋aspasesand anti cancer drags锛嶤ancerResearch锛1998锛58锛5315-5320Li 锛孏ri zi a Am brosi ni 锛孍m i l y Y锛嶤hu锛宔t a1锛嶤ontrol of apoptosi sand m i toti c29锛嶧engzhispi ndl e checkpoi nt by survi vi n锛嶯 ature锛1992锛396锛582—58430锛嶻ang L锛孋ao Z锛孻an H锛宔t al 锛孋oexi stence of hi gh l evel s 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